Scopus Research — Ataa Khalil Hussien

Gastrointestinal cancers, including stomach, liver, oesophageal, pancreatic, and colorectal cancers, represent more than a quarter of all cancers. Many abnormal gene expressions and dysregulated signalling pathways have been found in human cancer. Cancer often has activating mutations of the KRAS (Kirsten rat sarcoma virus) oncogene. Fifty blood samples from gastrointestinal cancer patients were gathered from the Merjan Teaching Hospital in Babylon, Iraq, and were used for a case-control study in the Oncology Center. According to the results, the most common cancers were found in the colon (29%), followed by the liver (27%), pancreas (19%), stomach (13%), and other (12%). In this work, we evaluated the distribution of KRAS mutations across the gastrointestinal tract. Sequencing data revealed a significant regional difference in the frequency of KRAS mutations, while the alignment results revealed the presence of six variations in the analysed samples when compared with the referring reference DNA sequences. Six highly interesting nucleic acid polymorphisms were detected in the investigated samples. When combined with additional carcinogenic markers such as the patient sex, age, consistent molecular subtypes, and tumour stage, KRAS mutation is not the deterministic carcinogenic factor for gastrointestinal malignancies. © 2024 by the authors. Licensee PHARMAKON-Press, Athens, Greece.

Using microorganisms to make this nanomaterial is a new research technique. In a culture medium, Trichophyton rubrum was permitted to biosynthesis silver nanoparticles. This study used Trichophyton rubrum, a dermatophytes fungus, to make silver nanoparticles. These species' clinical strains were produced in a medium containing mineral salt and cultured for 5-7 days at 25°C. Each culture's cell-free filtrate was taken and used to make AgNps in the presence of 1 mM AgNO3. The reduction of Ag+ ions in metal nanoparticles was virtually studied by observing the colour of the solution, which changed to a reddish-light brown after 72 hours. SEM was used to establish the presence of AgNo3. The presence of AgNPs was confirmed by SEM, which revealed that they are primarily spherical and 100nm in size. Furthermore, the findings showed that silver nanoparticles have antifungal activity against both infections in a concentration-dependent manner. At (150 ppm) of AgNPs, the growth decreased. Copyright © 2022 by.

Colorectal cancer is ranked to have high mortality among most malignancies worldwide. In the adult population, the seroprevalence rates of the John Cunningham virus (JCV) range from 70% to 90%. Recently the association for JCV in many malignant tumours have been reported worldwide, including colonic and rectum cancers. Interleukin-1β (IL-1β) can promote tumour growth where it is abundant in the tumour microenvironment, and its up-regulation is considered a poor prognostic feature in different types of solid tumours, including colon malignancies. One hundred tissue biopsies belonged to 50 patients with colorectal cancers and 30 benign colonic tumour patients, and 20 colorectal control tissues were enrolled in this study. JCV was detected via chromogenic in situ hybridization (CISH), while IL-1 beta was detected by immunohistochemistry (IHC). The recorded data showed that 21 out of 50 (42%) tissue samples with colorectal carcinoma showed positive CISH reactions for JCV DNA in this study. The benign colorectal tumours group revealed positive signals in 2 out of 30 tissues representing 6.7% of this group. Lastly, no control tissues showed positive signals for the JCV -CISH test. The positive signals of IL-1 Beta-IHC detection were found in 26 out of 50 (52 %) colorectal carcinoma tissues, while in the benign colorectal tumour was 43.3% (13 out of 30) and in AHC was 20% (4 out of 20 tissues). The high rates of JCV infection in this group of Iraqi patients with colonic adenocarcinoma in concordance with IL-1 Beta expression could play an essential role in the development and progression of these malignant tumours along with benign colonic tumours. To analyze the concordant expression of IL-1 beta gene and JCV in issues from a group of Iraqi patients with colonic adenocarcinomas. © 2022 Razi Vaccine and Serum Research Institute. All rights reserved.