The Role of Parasites in Detecting Biological Evidence in Forensic Investigations – Assist. Lect. Huwaida Nizal Hussein

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Parasites are biologically important organisms because of their ability to live inside or on the surface of other living organisms and cause various diseases affecting human and animal health. The importance of parasitology extends beyond medical diagnosis and treatment to forensic science, where parasites may serve as biological indicators that help investigators interpret certain forensic cases and understand associated health and environmental conditions. Forensic parasitology involves examining parasites and their developmental stages, including eggs, cysts, and larvae, in samples collected during investigations. These stages may be detected in fecal samples, blood, tissues, or environmental materials associated with crime scenes, providing useful information about infections or previous environmental exposure. Intestinal parasites are particularly relevant examples. Organisms such as Ascaris lumbricoides and Giardia duodenalis may be detected through their eggs or cysts in appropriate samples. Their presence may indicate exposure to contaminated food or water, although such findings cannot independently establish the exact location or timing of infection. Several laboratory techniques are used to detect parasites, including direct microscopic examination, concentration methods, and specialized staining procedures. Molecular diagnostic techniques, particularly Polymerase Chain Reaction (PCR), can also identify parasite genetic material and improve species-level identification. In forensic investigations, parasitological examinations may contribute to interpreting pathological findings or deaths potentially associated with severe parasitic infections. They may also provide supporting information when examining biological samples of uncertain origin, provided that contamination control and proper chain-of-custody procedures are maintained. In conclusion, forensic parasitology represents a valuable interdisciplinary field that connects biological sciences with forensic investigations. The examination of parasites can provide useful medical and environmental information and strengthen cooperation between diagnostic laboratories and forensic specialists.