Annals of R.S.C.B., ISSN: 1583-6258, Vol. 25, Issue 6, 2021, Pages. 1554-1560
Received 25 April 2021; Accepted 08 May 2021.
http://annalsofrscb.ro 1554
Association of Chronic Helicobacter pylori infection with Iron
Deficiency Anemia
Qusay Abdulazahraa Yaqoob1
, Haider Ali Mahdi2
, Hassan Ali Farman3
, Mohammed
Abdulzahra Sasaa4
1University of kufa/Iraq
2 Ministry of Health, Al-Sader Medical City, Al-Najaf, Iraq
3 Department of Anesthesia and intensive care techniques, college of Health & Medical Techniques, Al-Furat AlAwsat technical university, Iraq.
4Al-Mustaqbal University College, Anesthesia techniques department, Hillah, Iraq.

*Corresponding Author’s E-mail: [email protected]
Abstract- The study sample consisted of 120 participants divided equally into two groups; 60
patients with H.pylori infectin with iron defeciency anemia and 60 healthy individuals. Their age
ranged between 30–70 years, 27 of them were males and 33 were females. H. pylori patients
were recruited the Al Sadder Teaching Hospital and the Gastrointestinal Diseases center in AlNajaf AL-Ashraf city, through the duration of December 2019 till February 2020. All patients
diagnosed with H.pylori by clinical symptoms and confirmed by H. pylori stool antigen was
detected by strips. Verbal consent is taken from each patient before sample. Ten ml of blood are
withdrawn by vein puncture from H.pylori patients and healthy control. A tourniquet was applied
directly on the skin around the arm, the skin over the vein was sterilized with 70٪ ethyl alcohol,
then blood was collected divided into three part; one (2ml) transfer to EDTA tube for estimation
of CBC, part two (2ml) was put in ESR tube with anticoagulant and part three (4 ml) put gel tube
to be allowed to clot at room temperature for 15-20 minutes, and then it was centrifuged for 5
minutes at 5000xg , to get the serum, the serum was collected for estimation of iron, ferritin, AST
and ALT. The result shows that there were statistically significant differences at 0.05 in Hb level
between cases (m= 10.91) and controls (m= 14.26), P value 0.000, which means that Hb level
was significantly lower among cases compared to controls. In addition, there were statistically
non-significant differences at 0.05 in RBC between cases (m= 4.36) and controls (m= 4.55), P
value >0.05, which means that RBC were lower among cases compared to controls. In addition,
there were statistically significant differences at 0.05 in WBCs between cases (m= 9.76) and
controls (m=8.68), P value <0.05, which means that WBCs were significantly higher among
cases compared to controls. The results also shows that there were statistically significant
differences at 0.05 in PCV level between cases (m= 32.33) and controls (m= 43.01), P value
0.000, which means that PCV level was significantly lower among cases compared to controls.
Furthermore, there were statistically significant differences at 0.05 in MCV level between cases
(m= 63.18) and controls (m=83.56), P value 0.000, which means that MCV level was
significantly lower among cases compared to controls. On the other hand, there were statistically
insignificant differences between the two groups in levels of MCHC (P=>0.05). In addition, there
were statistically significant differences at 0.05 in MCH and PLT between cases and controls P
value 0.000. The result also shows that there were statistically significant differences at 0.05 in
serum iron, ferritin, AST and ALT level between cases and controls, P value 0.001. In addition,
there were statistically significant differences at 0.05 in ESR level between cases and controls P